Naturally occurring glycosidases in milk from native cattle breeds: activity and consequences on free and protein bound-glycans /

Anne Vuholm Sunds; Ida Schwartz Roland; Ulrik Kræmer Sundekilde; Martin Nørmark Thesbjerg; Randall Robinson; Apichaya Bunyatratchata; Maria Glantz; Marie Paulsson; Daiva Leskauskaite; Anne Pihlanto; Ragnhild Inglingstad; Tove Gulbrandsen Devold; Gerd Elisabeth Vegarud; Bryndis Eva Birgisdottir; Maria Gudjonsdottir; Daniela Barile; Lotte Bach Larsen; Nina Aagaard Poulsen

doi:10.3390/metabo11100662

Title	Naturally occurring glycosidases in milk from native cattle breeds: activity and consequences on free and protein bound-glycans /
Authors	Sunds, Anne Vuholm ; Schwartz Roland, Ida ; Kræmer Sundekilde, Ulrik ; Nørmark Thesbjerg, Martin ; Robinson, Randall ; Bunyatratchata, Apichaya ; Glantz, Maria ; Paulsson, Marie ; Leskauskaite, Daiva ; Pihlanto, Anne ; Inglingstad, Ragnhild ; Gulbrandsen Devold, Tove ; Vegarud, Gerd Elisabeth ; Birgisdottir, Bryndis Eva ; Gudjonsdottir, Maria ; Barile, Daniela ; Bach Larsen, Lotte ; Poulsen, Nina Aagaard
DOI	10.3390/metabo11100662
Full Text
Is Part of	Metabolites.. Basel : MDPI. 2021, vol. 11, iss. 10, art. no. 662, p. 1-24.. ISSN 2218-1989
Keywords [eng]	natural glycosidases ; oligosaccharides ; O-linked glycans ; principal component analysis ; cattle breeds ; mass spectrometry ; NMR spectroscopy
Abstract [eng]	Little is known about the extent of variation and activity of naturally occurring milk gly- cosidases and their potential to degrade milk glycans. A multi-omics approach was used to investi- gate the relationship between glycosidases and important bioactive compounds such as free oligo- saccharides and O-linked glycans in bovine milk. Using 4-methylumbelliferone (4-MU) assays ac- tivities of eight indigenous glycosidases were determined, and by mass spectrometry and 1H NMR spectroscopy various substrates and metabolite products were quantified in a subset of milk sam- ples from eight native North European cattle breeds. The results showed a clear variation in glyco- sidase activities among the native breeds. Interestingly, negative correlations between some glyco- sidases including β-galactosidase, N-acetyl-β-D-glucosaminidase, certain oligosaccharide isomers as well as O-linked glycans of κ-casein were revealed. Further, a positive correlation was found for free fucose content and α-fucosidase activity (r = 0.37, P-value < 0.001) indicating cleavage of fuco- sylated glycans in milk at room temperature. The results obtained suggest that milk glycosidases might partially degrade valuable glycans, which would result in lower recovery of glycans and thus represent a loss for the dairy ingredients industry if these activities are pronounced.
Published	Basel : MDPI
Type	Journal article
Language	English
Publication date	2021
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„Naturally occurring glycosidases in milk from native cattle breeds: activity and consequences on free and protein bound-glycans /“