| Title |
Enhancement of electrospun polycaprolactone scaffold biocompatibility / |
| Authors |
Dabašinskaitė, Lauryna ; Krugly, Edvinas ; Baniukaitienė, Odeta ; Čiužas, Darius ; Martuzevičius, Dainius |
| DOI |
10.22203/eCM |
| Full Text |
|
| Is Part of |
TERMIS European chapter meeting 2020: Broadening the targets and approaches for regenerative medicine, Manchester, UK, May 26 – 29, 2020.. [S.l.] : eCM. 2020, P84, p. 84.. ISSN 2522-235X |
| Keywords [eng] |
fibre technology ; polymers ; natural / synthetic / responsive |
| Abstract [eng] |
INTRODUCTION:Synthetic and natural polymers are used to fabricate scaffolds for tissue engineering. Synthetic scaffolds offer several advantages compared to scaffolds made from natural polymers, including longer shelf-life, cost efficacy, and limited risk of rejection. Polycaprolactone is one of the most suitable and desired synthetic biodegradable polymers having strong mechanical properties and biocompatibility. Although polycaprolactone has properties that are highly attractive for tissue engineering, the problem related with its hydrophobicity remains. In case of replicating the structure and properties of natural human tissues, electrospinning is one of the most popular method to manufacture the scaffolds. In order to adapt the polymeric fibre for tissue engineering, it is crucial to improve the hydrophilicity of the polymer and thus to create a suitable growth medium for cells. METHODS:The aim of this study was to fabricate polycaprolactone (PCL) construct with uniform morphology using solution electrospinning, analyse the O₃ in water treatment effect on PCL scaffold for cell growth and proliferation. Sample fibres (weigh 20 to 50 mg) were ozonated for the period of 30 to 120 minutes. Scaffold physicochemical properties and biocompatibility were measured using ATR-FTIR, XRD, water contact angle, number of carboxyl groups determination, hydrophilic analysis, mechanical analysis and cell proliferation evaluated by the CCK-8 assay. RESULTS:The ozone treatment resulted in formation of O-H group in the main chain of PCL scaffolds and improved the hydrophilicity. The duration of treatment had a positive effect on cell proliferation. After one day cell count of the longest O₃ duration treatment time was approximately 4 times greater compared to untreated scaffold. Although the cell count dropped substantially after 3 days (from 1.74 ± 0.02 to 1.08 ± 0.35), the remaining cells remained viable and proliferated for up to 14 days (0.49 ± 0.03). DISCUSSION & CONCLUSIONS:The O3 treatment of electrospun polycaprolactone scaffold demonstrated an improve on performance of cell growth, with potential applications in tissue engineering. |
| Published |
[S.l.] : eCM |
| Type |
Conference paper |
| Language |
English |
| Publication date |
2020 |
| CC license |
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